Durham, North Carolina

School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

 

Grant/sponsor funded [50% or more external]

 

The Wolf lab, in the Division of Nephrology at the Duke University School of Medicine, is seeking a highly motivated postdoctoral trainee to study disordered mineral metabolism in health and kidney diseases. The focus of Dr. Wolf’s research lies on disordered mineral metabolism across the spectrum of kidney disease from early stages to end-stage renal disease and following kidney transplantation. The lab primarily studies the role of fibroblast growth factor 23 (FGF23) in phosphate and calcium homeostasis in health and in disease. The team established the concept that excess FGF23 increases risk of cardiovascular disease and death through their clinical trials, patient-oriented, epidemiological, and laboratory-based research. Since 2002, Dr. Wolf’s research has been supported by the American Heart Association, National Kidney Foundation, American Society of Nephrology, and National Institutes of Health. We offer an inspiring intellectual, collaborative, and multidisciplinary research environment to support your career goals and provide access to state-of-the-art facilities.

 

The successful candidate will investigate the tissue-specific effects of the vitamin D degrading enzyme, CYP24A1, in health and in disease. The Wolf lab provides intensive research training in in vivo mouse genetics, organoid and cell culture assays, and pathophysiology of kidney diseases. The Postdoctoral fellow is expected to work independently but in close cooperation with the principal investigator, Dr. Myles Wolf. The postdoc will also work with other collaborating Research Faculty, laboratory staff, and postdoctoral fellows to perform routine and complex laboratory procedures throughout the training period. Investigations will be performed using gene-modified mice, preclinical models, and cell culture and organoid studies. Interested individuals with a diverse prior research background including molecular and cell biology, metabolism, physiology, or a related field are encouraged to apply to this position. This project will require strong hands-on experience in in vivo studies using mouse as a model organism for human disease. Experience with gene-modified mouse models is preferred. The position is funded by an NIH grant and is available immediately.

 

Primary Duties and Responsibilities

  1. Research using vertebrate animals, specifically mice, to study kidney function and cardio-vascular function.
  • Participate in the design of complex breeding and experimental design for gene-modified mouse lines
  • Plan and execute complex, multi-stepped animal studies.
  • Perform animal procedures for physiological studies including:
    • Intravenous, intra-CSF, and intraperitoneal injections to mice.
    • Survival surgeries in mice in relevant disease models, including models of acute kidney injury (AKI) and chronic kidney disease (CKD).
    • Post-operative care of animals.
    • Non survival surgeries, tissue harvest, and organ and specimen collection and processing.
    • Animal handling skills and techniques.
    • Various methods for blood and urine collection
  1. Research in a biomedical laboratory
  • Perform techniques including PCR, rt-qPCR, Western blotting, cell culture, ELISAs, and gel electrophoresis, and preparation and staining of histological specimens. 
  • Perform experimental protocols and procedures, including detailed data collection, and analysis, operation and maintenance of specialized equipment and maintain precise records of experimental work.
  • Maintain precise records of experimental work.
  • Participate in bi-weekly lab meetings with PI and research team to review and discuss data and access study progress.
  1. Other professional activities related to research
  • Scientific writing and communication.
  • Ability to critically review scientific literature.
  • Participate in publications and presentations as first-author or co-author.
  • Participate in the preparation of preliminary data, graphs and figures for grant proposals.

 

Interested candidates should submit the following documents to Dr. Myles Wolf (myles.wolf@duke.edu)

1) A cover letter that includes a short summary of interests

2) CV with 3 reference contacts.

 

Minimum Qualifications

 

Education

See job description for education requirements.

 

Experience

See job description for requirements.

 

 

The mission of the Division Nephrology in the Department of Medicine is to combine the delivery of outstanding clinical care with cutting-edge research and innovative teaching. To this end, the programs in the division are structured to address the most pressing needs facing patients with kidney diseases. See https://medicine.duke.edu/divisions/nephrology/about-duke-nephrology for more information.

 

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

 

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

 

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

 

Durham, North Carolina

As a world-class academic and health care system, Duke Health strives to transform medicine and health locally and globally through innovative scientific research, rapid translation of breakthrough discoveries, educating future clinical and scientific leaders, advocating and practicing evidence-based medicine to improve community health, and leading efforts to eliminate health inequalities.

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