Bridgewater Township, New Jersey

Job Description Summary:

The Research Associate II, Gene Therapy ( AAV Production) is responsible for conducting scientific experiments that contribute to research and drug discovery activities at PTC. This position assists in production, purification, and characterization of recombinant adeno-associated viral (AAV) vectors to support in vivo efficacy and safety study in animals, as well as in the optimization of current AAV production methodology. The incumbent is responsible for communicating experimental results to his/her supervisor and the project team.

The Research Associate II, Gene Therapy ( AAV Production) supports adherence to all regulatory requirements and company Standard Operating Procedures (SOPs) including as appropriate.

Job Description:


Primary duties/responsibilities:
  • Under general guidance, executes experiments and communicates results as part of multi-disciplinary team(s).
  • Applies relevant scientific principles and techniques to research problems.
  • Maintains detailed laboratory notebooks.
  • Maintains knowledge of state-of-the-art principles and techniques.
  • Ability to trouble shoot experiments -designing controls, generating interpretable data.
  • Presents findings internally and contributes to the publication of the work of the project team.
  • Contributes to the development of patent applications as appropriate.
  • Complies with all safety requirements.
  • Contributes to laboratory needs, such as stock room maintenance and ordering supplies.
  • Involved in general lab duties as assigned.

Specific duties may also include, but may not be limited to, the following:
  • Performs production of rAAV vectors for in vivo efficacy and pharmacology studies.
  • Provides support to the team in the characterization of the produced rAAV vectors, including silver staining for purity, ELISA for capsid copies and serotype, transduction & Western blotting for MOI, alkaline gels and SEC-MALS for titer and full/empty ratio.
  • Supports development of new methods and optimization of the existing methods for AAV production in-house.
  • Provides support for in vivo studies as required.


* Minimum level of education and years of relevant work experience.
  • Bachelor's degree in a Biology scientific discipline (Molecular Biology or Biochemistry or Cell Biology) with a minimum of 1-2 years' relevant experience.

* Special knowledge or skills needed and/or licenses or certificates required.
  • Excellent aseptic techniques with mammalian cell culture maintenance and cryopreservation experience.
  • In depth knowledge of Cell Biology and Molecular Biology. Knowledge of and ability to use standard molecular and cell biology techniques including DNA and RNA purification, quantification and transfection, molecular cloning, PCR, RT-PCR, qPCR.
  • Experience in ELISA, Western Blot.
  • Experience with data analysis.
  • Ability to independently execute experimental assays.
  • Demonstrates technical proficiency, and the ability to collaborate with others on cross functional teams in a fast-paced environment consisting of internal and external team members.
  • Possesses knowledge of scientific principles and concepts and keeps current with relevant scientific literature.
  • Proficiency with Microsoft Office- preferably MS Excel and MS PowerPoint.
  • Effective verbal and written communication and skills.
  • Analytical thinker with problem-solving skills and the ability to adapt to changing priorities and deadlines.
  • Effective planning, organization and time management skills including the ability to support and prioritize multiple projects.

*Special knowledge or skills and/or licenses or certificates preferred.
  • Knowledge of AAV methodology highly desired.
  • Working knowledge of NGS sequencing is a plus.
  • Ability to work with LIMS and databases.

* Travel requirements

Up to 5%

*Physical requirements (only note if the job requires physical capacity beyond general office work)

Office/Laboratory based position

**At PTC Therapeutics we are seeking candidates in the United States who are fully vaccinated against COVID-19 or who will be fully vaccinated by the time they begin the position.

EEO Statement:

PTC Therapeutics is an equal opportunity employer. We welcome applications from all individuals, regardless of race, color, national origin, gender, age, physical characteristics, social origin, disability, religion, family status, pregnancy, sexual orientation, gender identity, gender expression, disability, veteran status or any unlawful criterion under applicable law. We are committed to treating all applicants fairly and avoiding discrimination.

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Bridgewater Township, New Jersey

PTC is a science-driven, patient-centered biopharmaceutical company focused on discovering, developing & commercializing medicine for patients with rare disease.

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