RESEARCH TECHNICIAN I - Infectious Diseases - Military Veterans

at Duke University and Duke University Health System

Durham, North Carolina

School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.




Job Summary


Dr. Thuy Le, MD, PhD, is looking for a curious, motivated junior researcher, with an interest in infectious diseases and molecular biology, to join her team as a research technician. The individual will be working on a project focused on dissecting the molecular mechanism for virulence of Talaromyces marneffei, a fungal pathogen endemic in Southeast Asia that is a leading cause of death in patients with advanced HIV disease.  The individual will engage in gene deletion experiments using homologous recombination through Agrobacterium medicated trans-kingdom conjugation, and will assess any correlations of gene deletion and functional consequences including hypermutation phenotype, fungal burden, tissue pathology, and survival in mice. This work will involve a combination of both guided and independent execution of standardized molecular and microbiology techniques, experiments, and the support of research-oriented activities at Duke University Department of Medicine, Division of Infectious Disease. The researcher will have the opportunity to master multiple laboratory techniques, include ELISAs, real-time PCR assays, fungal culture, cloning, genetic transformation. In addition to the laboratory and animal experiments, the person will have weekly contact with Dr. Le and receive a high level of mentorship.


Interested individuals are encouraged to contact Dr. Le with any further queries before and during the application process at


Duties and Responsibilities


Able to conduct a variety of experimental investigations with minimum guidance – knowledge and experience in basic microbiology, molecular biology, immunology techniques are desirable. Prior experience in handling animal (mice) experiments are particularly desirable.


Willing to learn new procedures, including handling and manipulation of laboratory mice, tissue section, staining, qPCR, and immunoassays. Good Laboratory Practice is essential.


Keep excellent laboratory records of experiments performed. Meet with PI weekly to provide updates and discuss the interpretation of experimental results.


Assist the Laboratory Manager with managing laboratory functions, including - maintaining inventory of reagents and consumables.


Maintain laboratory – including care of equipment, arranging waste disposal, storage of lab supplies.


Perform other related duties incidental to the work described herein.


Good inter-personal skills and a team player in the laboratory environment.


Minimum Qualifications



Work requires an Associate's degree in botany, biology, zoology, psychology or other science related scientific field.





Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.


Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.


Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.


Durham, North Carolina

As a world-class academic and health care system, Duke Health strives to transform medicine and health locally and globally through innovative scientific research, rapid translation of breakthrough discoveries, educating future clinical and scientific leaders, advocating and practicing evidence-based medicine to improve community health, and leading efforts to eliminate health inequalities.

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