Durham, North Carolina

This Position is Fully Grant Funded

School of Medicine:


Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.


Comprised of 2,400 faculty physicians and researchers, the Duke University School of Medicine along with the Duke University School of Nursing and Duke University Health System create Duke Health. Duke Health is a world-class health care network. Founded in 1998 to provide efficient, responsive care, the health system offers a full network of health services and encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.








The Azoitei Lab at the Duke Human Vaccine Institute (DHVI), is currently recruiting for a Lab Analyst I to join our team. DHVI is an interdisciplinary, interdepartmental institute dedicated to the study of basic and translational science required to understand host-pathogen interactions that can be translated to vaccines against human diseases. We are a team of highly interactive investigators that have expertise in immunology, molecular biology, virology, microbiology, structural biology, computational biology, and vaccine science.


Research in the Azoitei lab is focused on two synergistic areas that are critical for the development of vaccines against highly infectious pathogens such as HIV, influenza and SRAS-CoV-2: 1) rational immunogen design for the induction of broadly neutralizing antibodies and 2) determining the biophysical properties of antigens that lead to B cell activation and development for antibody secretion. For immunogen design, we employ a rational, structure-based approach that utilizes state of the art computational protein design algorithms (Rosetta) and high-throughput library screening on the surface of yeast and mammalian cells. We are currently developing immunogens against HIV, influenza and SARS-CoV-2. To study B cell signaling in response to antigens, we utilize molecular tools (fluorescent biosensors  and optogenetic probes) to visualize and control the activity of key signaling molecules in live cell microscopy experiments. While the molecules involved in B cell activation are well characterized, little is known about how the subcellular localization, duration and intensity of their activities are shaped by the biophysical properties of different antigens. All our work involves collaborations with structural biologists, immunologists and virologists. 



  • Independently conduct a variety of duties in the planning, development and implementation of high throughput library screening experiments, such as development of DNA libraries using molecular biology techniques and screening on the surface of  yeast and mammalian cells by FACS, followed by analysis of Next Generation Sequence data.
  • Independently perform recombinant protein expression and purification from mammalian and bacterial cultures, including metal affinity purification and Size Exclusion Chromatography.
  • Perform tissue culture and propagation of mammalian cell lines.
  • Evaluate and analyze collected data and prepare reports, analyses, and presentations. Develop charts and graphs for scientific publications.
  • Evaluate scientific literature and recommend new techniques and experimental approaches. 
  • Perform general laboratory management (laboratory supply ordering, maintaining biosafety protocols, etc.). Manage laboratory databases and folders and liaison with IT.
  • Work with various investigators, research personnel and staff to solve specific operating problems and improve technical activities. Confer with principal investigator in developing plans for research projects and to discuss the interpretation of results and the preparation of manuscripts for publication.
  • Perform ordering responsibilities for the laboratory, including taking inventory, restocking, submitting orders, managing samples and coordinating with other lab members regarding their specific research needs
  • Perform other laboratory or data management duties, including laboratory maintenance tasks, as requested by the Principal Investigator

Required Qualifications at this Level

  • Education/Training: Work requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.
  • Experience: Work requires two years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE



Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.


Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.


Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.


Durham, North Carolina

As a world-class academic and health care system, Duke Health strives to transform medicine and health locally and globally through innovative scientific research, rapid translation of breakthrough discoveries, educating future clinical and scientific leaders, advocating and practicing evidence-based medicine to improve community health, and leading efforts to eliminate health inequalities.

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