Scheduled Hours 40 Position Summary This position assists with the technical aspects of studies and experiments, including documentation and preparation of materials. The Kulkarni lab, is a NIH-funded laboratory in the Division of Pulmonary and Critical Care Medicine at Washington University. We are seeking a research technician II to investigate how innate immune responses, such as the complement system, modulate the severity of lung injury. The overarching goal of our research program is to determine how epithelial cell-derived proteins can be harnessed to mitigate the risk of acute lung injury in various settings, to ultimately reduce the burden of end-stage lung disease. We have active projects investigating lung injury due to bacterial infections (such as Pseudomonas aeruginosa and Staphylococcus aureus), and the short- and long-term consequences of ischemia-reperfusion injury occurring in the context of lung transplantation. We utilize state-of-the-art technical modalities, including flow cytometry, ELISA, protein and DNA-based molecular biology, biochemistry, cell/tissue culture, fluorescence and confocal microscopy, and DNA/RNA sequencing. The Research Technician will be responsible for both conducting experiments, including animal experiments, and developing and executing the plans in the laboratory, in partnership with the principal investigator. A major focus of our work involves distinguishing the role of locally-derived complement proteins in the lung from those present in the blood, and how they modulate the development of pneumonia and acute lung injury. We use multiple in vitro and in vivo approaches to dissect the mechanism by which these proteins contribute to cellular survival. We have a robust collaboration with our Airway Epithelial Cell Core, where in we work with primary tracheobronchial cells, and the Department of Surgery, with whom we investigate models of experimental lung transplantation. Additionally, we draw upon a robust biorepository of lung tissue, bronchoalveolar lavage fluid and DNA from lung transplant recipients to guide and validate our research. Job Description For additional details, please visit https://sites.wustl.edu/kulkarnilab . Primary Duties & Responsibilities Assists with research studies, experiments and assays, including collection of data, preparation of solutions and set-up and maintenance of equipment. Performs data entry and maintains data files on research. Prepares tissue cultures, reagents, probe tests, etc. Complies with established safety procedures and maintains required documentation on laboratory and specimen conditions. Ensures lab conditions and equipment are properly cleaned and maintained in accordance with established procedures. Assists with general lab maintenance and cleaning. Performs other duties as assigned. Working Conditions This position works in a laboratory environment with potential exposure to biological and chemical hazards.Â The individual must be physically able to wear protective equipment and to provide standard care to research animals. Preferred Qualifications Demonstrated ability to work independently and communicate with laboratory staff and investigators.Â Ability to maintain complete and organized records/reports.Â Working knowledge of computer software and general lab equipment. Required Qualifications Bachelorâ™s degree and up to one year of experience in a laboratory setting or an equivalent combination of education and experience equaling four years. Grade R06-H Salary Range $15.61 - $24.39 / Hourly The salary range reflects base salaries paid for positions in a given job grade across the University. Individual rates within the range will be determined by factors including one's qualifications and performance, equity with others in the department, market rates for positions within the same grade and department budget. Pre-Employment Screening All external candidates receiving an offer for employment will be required to submit to pre-employment screening for this position. The screenings will include criminal background check and, as applicable for the position, other background checks, drug screen, an employment and education or licensure/certification verification, physical examination, certain vaccinations and/or governmental registry checks. All offers are contingent upon successful completion of required screening. Benefits Statement Washington University in St. Louis is committed to providing a comprehensive and competitive benefits package to our employees. Benefits eligibility is subject to employment status, full-time equivalent (FTE) workload, and weekly standard hours. Please visit our website at https://hr.wustl.edu/benefits/ to view a summary of benefits. EEO/AA Statement Washington University is an equal opportunity and affirmative action employer. All qualified applicants will receive consideration without regard to an individualâ™s sex, race, color, religion, age, disability status, protected veteran status, national or ethnic origin, gender identity or expression, sexual orientation. Women, minorities, protected veterans and the disabled are strongly encouraged to apply. 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