PostDoctoral Associate in Biophysics and Structural Biology | Rutgers University - Military Veterans

at HERC - New Jersey/Eastern PA/Delaware

New Brunswick, New Jersey

Position Summary: A postdoctoral position in cryo-electron microscopy (cryo-EM) and single-molecule studies of DNA repair and replication is available in the laboratory of Dr. Arek Kulczyk in the Institute for Quantitative Biomedicine at Rutgers University. Kulczyk lab ( integrates structural approaches, in particular single-particle cryo-EM, single-molecule methods and novel correlative light and electron microscopy ( CLEM ) techniques to study DNA replication and repair (Kulczyk et al., 2017, PNAS , 114:1848; Duderstadt et. al., 2016, Mol Cell, 64:1035; Geertsema et al., 2014, PNAS , 111:4073; Kulczyk et al., 2012, PNAS , 109:9408; Loparo et al., 2011, PNAS , 108:3584). Minimum Education and Experience: Applicants must hold a PhD in biophysics or in a related field and have a deep working knowledge of structural biology. Required Knowledge, Skills, and Abilities: Proficiency in cloning, protein expression, protein purification, and characterization of protein-nucleic acid complexes using a variety of biophysical methods (for example: BIAcore, ITC , AUC , DLS and NMR ). Research experience in cryo-electron microscopy (cryo-EM) and single-molecule biophysics is desirable, however not required. The selected candidate will obtain appropriate training in microscope alignments, data acquisition, image processing and structure calculation using a variety of cryo-EM software. The candidate should be proficient in Linux. Working knowledge of C++, Python, CUDA and experience in high performance computing ( HPC ) is desirable, but not required. Strong written and oral communication skills in English, as well as the ability to work independently and prepare scientific manuscripts are required. Equipment Utilized: The Institute for Quantitative Biomedicine houses the state-of-the-art cryo-EM facility (Thermo Fisher Scientific Talos Arctica cryo-electron microscope equipped with a Summit K2 direct electron detector, BioQuantum energy filter and a Volta phase plate; Leica DM6 FS/EM cryo- CLEM system), NMR facility (Bruker 800 MHz and 700 MHz with cryoprobes and two 600 MHz spectrometers), mass spectrometry and super-resolution imaging facilities, and is the world headquarters of the Research Collaboratory for Structural Bioinformatics ( RCSB ) consortium and the Protein Data Bank ( PDB ). In addition, the effort to purchase Thermo Fisher Scientific Titan Krios and dual-beam FIB - SEM instruments is under way. Posting Number: 18FA0754 Location: Busch (RU-New Brunswick)

New Brunswick, New Jersey

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